Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science Regular Papers
Evaluation of Superoxide Anion Radicals Generated from an Aqueous Extract of Particulate Phase Cigarette Smoke by Electron Spin Resonance Using 5,5-Dimethyl-1-pyrroline-N-oxide
Yuichiro TAKANAMITsutomu NAKAYAMA
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2011 Volume 75 Issue 1 Pages 34-39

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Abstract

The reactive oxygen species generated by an aqueous extract of the particulate phase of cigarette smoke were evaluated by an electron spin resonance (ESR) analysis, using spin-trapping agents, and by comparing with model reaction systems. The ESR signals of DMPO-OH were detected from the extract by using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). These signals were eliminated by adding superoxide dismutase, but hardly by catalase. These responses of the ESR signals to the scavengers were similar to those of a hypoxanthine-xanthine oxidase system. The results indicate that the signals of DMPO-OH from the extract were derived from a reaction product of DMPO with superoxide anion radicals and clarify the mechanism by which the extract generated superoxide anion radicals.

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© 2011 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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