Characterization of Recombinant β-Amylases from Oryza sativa

Tomojiro KOIDE; Yasuo OHNISHI; Sueharu HORINOUCHI

doi:10.1271/bbb.100872

Bioscience, Biotechnology, and Biochemistry

Online ISSN : 1347-6947
Print ISSN : 0916-8451

Biochemistry & Molecular Biology Notes

Characterization of Recombinant β-Amylases from Oryza sativa

Tomojiro KOIDE, Yasuo OHNISHI, Sueharu HORINOUCHI

Author information

Keywords: Oryza sativa, β-amylase, recombinant protein, heat stability

JOURNAL FREE ACCESS

2011 Volume 75 Issue 4 Pages 793-796

DOI https://doi.org/10.1271/bbb.100872

View "Advance Publication" version (April 22, 2011).

Details

Abstract

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5–6.0, but they exhibited inferior heat stability to soybean β-amylase.

Content from these authors

This article cannot obtain the latest cited-by information.

Favorites & Alerts

Corresponding author

Register with J-STAGE for free!