Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Regular Papers
A Secondary Structure in the 5' Untranslated Region of adhE mRNA Required for RNase G-Dependent Regulation
Kazutaka ITOKohshin HAMASAKIAya KAYAMORIPhuong Anh Thi NGUYENKaoru AMAGAIMasaaki WACHI
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2013 Volume 77 Issue 12 Pages 2473-2479

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Abstract

Escherichia coli RNase G is involved in the degradation of several mRNAs, including adhE and eno, which encode alcohol dehydrogenase and enolase respectively. Previous research indicates that the 5' untranslated region (5'-UTR) of adhE mRNA gives RNase G-dependency to lacZ mRNA when tagged at the 5'-end, but it has not been elucidated yet how RNase G recognizes adhE mRNA. Primer extension analysis revealed that RNase G cleaved a phosphodiester bond between −19A and −18C in the 5'-UTR (the A of the start codon was defined as +1). Site-directed mutagenesis indicated that RNase G did not recognize the nucleotides at −19 and −18. Random deletion analysis indicated that the sequence from −145 to −125 was required for RNase G-dependent degradation. Secondary structure prediction and further site-directed deletion suggested that the stem-loop structure, with a bubble in the stem, is required for RNaseG-dependent degradation of adhE mRNA.

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© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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