A Rapid Method of Screening Lactic Acid Bacterial Strains for Conjugated Linoleic Acid Production

Gustavo A. ROMERO-PÉREZ; Ryo INOUE; Kazunari USHIDA; Takaji YAJIMA

doi:10.1271/bbb.120709

Microbiology & Fermentation Technology Notes

A Rapid Method of Screening Lactic Acid Bacterial Strains for Conjugated Linoleic Acid Production

Gustavo A. ROMERO-PÉREZ, Ryo INOUE, Kazunari USHIDA, Takaji YAJIMA

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Keywords: lactic acid bacteria, screening efficiency, linoleic acid tolerance, potassium phosphate buffer, conjugated linoleic acid

JOURNAL FREE ACCESS

2013 Volume 77 Issue 3 Pages 648-650

DOI https://doi.org/10.1271/bbb.120709

View "Advance Publication" version (March 7, 2013).

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Abstract

A more rapid and simpler method than those currently used was developed to screen conjugated linoleic acid (CLA)-producing bacteria isolated from cow milk. The screening of 500 strains was completed in 10 d and the screening efficiency was 10%. One strain resembling a Lactobacillus paracasei strain and two resembling L. helveticus strains converted free linoleic acid to total CLA ≥85%.

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