Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Construction of an Endogenous Selectable Marker Gene for the Rice Straw Degrading White-Rot Basidiomycete Cyathus stercoreus
Author information
JOURNALS FREE ACCESS Advance online publication
Supplementary material

Article ID: 120912

This article has a public article.

Cyathus stercoreus has a superior ability to increase the cellulase saccharification yields of rice straw under biological pretreatment. As a first step to improve the biological pretreatment efficiency of C. stercoreus further, a new gene transformation system was constructed. Uracil auxotrophic mutant uv-180 was generated as host strain by ultraviolet radiation. It was transformed using plasmid pGE containing the orotate phosphoribosyltransferase (CsURA5) and enhanced green fluorescent protein (egfp). Many transformants exhibited strong fluorescence, indicating successful genetic transformation. An intron was needed for the expression of egfp. The EGFP fluorescence intensities of the three transformants did not decay even after subculturing on uracil-containing semisolid medium 5 times, suggesting that the transformed plasmids were stably retained in the absence of selective pressure.

Information related to the author

This article cannot obtain the latest cited-by information.

© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry