Studies on Cycasin, a New Toxic Glycoside, of Cycas revoluta Thunb

Part V. Quantitative Determination of Cycasin in Gycad Seeds

Abstract

Quantitative paper chromatography of cycasin is described, where the cycasin and sugars separated on the paper are eluted out and determined, according to the colorimetric micro analysis of sugars by Plumel. The contents of cycasin and free sugars contained in both premature and matured cycad seeds determined by this method are presented.