Abstract
Various attempts were made to accumulate 5 (4)-amino-4 (5)-imidazolecarboxamide (abreviated as AICA; in this paper, separate analyses for the riboside forms were not attempted, and hence AICA and AICA-riboside will be presented as total AICA) in a shaking culture medium by Escherichia coli strain B. The accumulation of non-acetylatable, diazotizable amines was accomplished by the addition of 0.01% of sulfadiazine, 0.2% of glucose, and 2% of peptone in the medium for sixteen hours at 30°C. E coli strain B was able to accumulate the amines in the pepton medium, even when glucose and the sulfonamide inhibtor were omitted. Although paper chromatographic and spectrophotometric analyses proved the accumulation of AICA and AICA-riboside by E. coli train B in the medium, another substance colored by the Bratton and Marshall method was also accumulated.
