Abstract
As reported previously promoting compounds for growth of Lactobacillus leichmannii 7830 in the extract of chick embryo treated at pH 12-13 with hot diluted alkali consisted of pyrimidine deoxyribosides (deoxycytidine, deoxyuridine, and thymidine) and unidentified compound. The compounds fractionated by paper chromatography from unidentified peak seem to be cytosine deoxyribonucleotide and thymine deoxyribonucleotide respectively by high voltage paper electrophoresis and paper chromatography. Namely, fractionated two compounds migrated toward the anode at pH 3.6, growth promoting zones for L. leichmannii agreed with spots by the cysteine-sulfuric acid reaction and Wade reaction, and both com-pounds were stable to hydrolysing at pH 2, 100°C for 5 minutes. The products by alkaline phosphatase from both compounds were identified to be thymidine and deoxycytidine by comparison of the relative mobilities with those of authentic deoxyribosides and by paper chromatographic analysis of pyrimidine deoxyribosides.
