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Agricultural and Biological Chemistry
Vol. 30 (1966) No. 2 P 164-168

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http://doi.org/10.1271/bbb1961.30.164


A thin-layer chromatographic method is described for the separation, identification and determination of 2, 4-dinitrophenyl sulfides. The derivatives are easily obtained from mercaptan and dinitrofluorobenzene in the presence of sodium bicarbonate at room tem-perature. The sulfides are separated on silica gel plates using a solvent mixture of benzene-xylene-carbon tetrachloride (2:1:1, v/v). The individual sulfides are determined spectrophotometrically, at 330_??_335mμ in ethanol, εmax ca. 13, 000, after washing out the plate with hexane and extraction from the adsorbent with acetone.

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