Separation and Determination of 2, 4-Dinitrophenylsulfides by Thin-layer Chromatography

Abstract

A thin-layer chromatographic method is described for the separation, identification and determination of 2, 4-dinitrophenyl sulfides. The derivatives are easily obtained from mercaptan and dinitrofluorobenzene in the presence of sodium bicarbonate at room tem-perature. The sulfides are separated on silica gel plates using a solvent mixture of benzene-xylene-carbon tetrachloride (2:1:1, v/v). The individual sulfides are determined spectrophotometrically, at 330_??_335mμ in ethanol, εmax ca. 13, 000, after washing out the plate with hexane and extraction from the adsorbent with acetone.