Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Milk Clotting Enzyme from Microorganisms
Part IV Immunological Studies on the Enzyme Properties
Shinjiro IWASAKITsuneo YASUIGakuzo TAMURAKei ARIMA
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1967 Volume 31 Issue 12 Pages 1427-1433

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Abstract
The antiserum against the milk clotting enzyme from Mucor pusillus reacted with the purified and crude enzyme preparations in precipitin test and inhibited their enzyme activities, but did not react with other enzymes such as rennin, pepsin, acid proteases from Aspergillus saitoi and Aspergillus oryzae, or the culture filtrates of some strains of Mucor and Rhizopus.
The antigen-antibody reaction was so specific that it might be possible with this antibody to identify this enzyme and also the strain itself.
Normal sera from some mammals inhibited this enzyme activity too, but the degree was less than that with rennin.
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