抄録
Streptomycin-phosphorylating enzyme was reported previously to be produced in my-celium of Streptomyces griseus HUT 6037 at late stage of growth. In the present investi-gation, this enzyme was purified 200 times as high in specific activity as cell-free extract by means of salting out, chromatography on DEAE-Sephadex A-25 and gel filtration with Sephadex G-100. This enzyme was most stable at pH 8.0 and required 10-2M Mg2+ in the reaction mixture for the highest activity. It lost the activity by heat treatment at 40°C for 15 min in absence of the substrate.
Mutant cultures were prepared on productivity of or tolerance to streptomycin, and their capacity to produce streptomycin-phosphorylating enzyme was examined. The cul-tures which had low to no capacity to produce streptomycin produced a small amount to none of the enzyme, suggesting that production of the streptomycin-phosphorylating enzyme had some correlation with streptomycin productivity of the culture. But no definite cor-relation was observed between productivity of the enzyme and the capacity to tolerate streptomycin.
