Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
L-Tryptophan Production by Analog-resistant Mutants Derived from a Phenylalanine and Tyrosine Double Auxotroph of Corynebacterium glutamicum
Hiroshi HAGINOKiyoshi NAKAYAMA
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1975 Volume 39 Issue 2 Pages 343-349

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Abstract

Mutants producing a large amount of L-tryptophan were derived from a phenylalanine and tyrosine double auxotroph of Corynebacterium glutamicum, KY 9456 which produced only a trace amount of L-tryptophan (150 μg/ml) and anthranilate. A mutant, 4 MT-11, resistant to 5-methyltryptophan, tryptophanhydroxamate, 6-fluorotryptophan and 4-methyltryptophan which was derived by stepwise mutagenic treatments produced L-tryptophan at a concentration of 4.9mg/ml in a molasses medium containing 10% of sugar calculated as glucose. L-Tryptophan production with 4 MT-11 was inhibited by L-phenylalanine and L-tyrosine. Accordingly, mutants resistant to phenylalanine and tyrosine analogs such as p-fluorophenylalanine, p-aminophenylalanine, tyrosinehydroxamate, and phenylalaninehydroxate were derived from 4 MT-11. One of the mutants, thus obtained, Px-115-97 produced L-tryptophan at a concentration of 12.0mg/ml in the molasses medium. L-Tryptophan production with the mutant was still sensitive to L-phenylalanine and L-tyrosine.

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