Reaction between Nitrite and Low Salt-soluble, Diffusible Fraction of Meat. Partial Purification and Some Properties of Unknown Reaction Products

Abstract

The reaction products between ¹⁵N-nitrite and 0.05M NaCl-soluble fraction of meat were chromatographed on Sephadex G-15 column. The unidentified ¹⁵N compound(s) which correspond to about 25% of added nitrite-¹⁵N eluted as one peak, and separated from peptides, nitrite and nitrate. These compounds had a tendency to be adsorbed to Sephadex gel, and eluted after nitrite and nitrate. They had no UV absorption from 240 to 320nm, and were not nucleotides, amino acids or peptides. They were subjected to thin-layer chromatography and paper electrophoresis The findings of paper electrophoresis showed that they were strongly acidic compounds.