Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Metabolism of Glycolate in Mitochondria of Euglena gracilis
Akiho YOKOTAYoshihisa NAKANOShozaburo KITAOKA
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1978 Volume 42 Issue 1 Pages 121-129

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Abstract

Difference spectra taken at -196°C were given to confirm that glycolate oxidation by Euglena mitochondria is linked to the electron transport system with the production of ATP. In feeding experiments with 1-14C-glycolate on E. gracilis cells having glycolate dehydrogenase only in mitochondria, greater parts of the taken-up radioactivity were excreted as 14CO2 and greater parts of the remaining were distributed to the amino acid and protein fractions, mainly as glycine and serine. Presence of ammonium decreased 14CO2 evolution and augmented incorporation and distribution to protein of label. Exhaustion of nitrogen source stopped glycolate uptake. Deficiency of nitrogen source repressed glycolate dehydrogenase most extensively among the enzymes involved in the glycolate pathway. The mechanism of regulation of the glycolate metabolism by nitrogen source is discussed.

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