Abstract
Isoamyl acetate was enzymatically synthesized from acetyl-CoA and isoamyl alcohol with the cell-free extract from Cladosporium cladosporioides No. 9. The acetic-ester synthesi-zing enzyme was fractionated from the cell-free extract by procedures including (NH4)2SO4 fractionation, gel filtration on Sephadex G-150, and column chromatography on DEAE-Sephadex A-50. This enzyme was homogeneous on SDS gel electrophoresis and its molecular weight was approximately 22, 000. The enzyme was obtained in about 50-fold purification in specific activity over that of the cell-free extract. The enzyme was most active at pH 6.0 and 25°C, and was relatively stable between pH 6.0 and 7.5.
