Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
High Efficiency of Heterologous Transformation of a Restrictionless and Modificationless Mutant of Bacillus subtilis 168
Takayuki HOSHINOTakeshi UOZUMITeruhiko BEPPUKei ARIMA
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1980 Volume 44 Issue 3 Pages 621-623

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Abstract

Efficiencies of transformation with heterologous DNAs were examined on B. subtilis 168-YSI1 and its restrictionless and modificationless mutant B. subtilis RM125. Whereas the efficiencies of homologous transformation on YS11 and RM125 were almost the same, RM125 showed 30-200 fold higher efficiencies than YS11 when heterologous DNAs were used as transforming DNAs.

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© Japan Society for Bioscience, Biotechnology, and Agrochemistry
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