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Agricultural and Biological Chemistry
Vol. 46 (1982) No. 11 P 2685-2689



Phenylalanine ammonia-lyase, trans-cinaamate 4-monooxygenase, and capsaicinoid synthetase [Agric. Biol. Chem., 44, 2907 (1980)] activities were investigated in the subcellular fractions from protoplasts of placenta of Capsicum fruits. The subcellular distribution of intermediates of the capsaicinoid biosynthesis, trans-cinnamic acid and trans-p-coumaric acid, and capsaicinoid were also investigated. The activity of trans-cinnamate 4-monooxygenase and capsaicinoid synthetase was in the vacuole fraction. While the activity of phenylalanine ammonia-lyase was in the cytosol fraction. After feeding L-[U-14C]phenylalanine to the protoplast, the newly synthesized trans-p-coumaric acid and capsaicinoid were found in the vacuole fraction, while trans-cinnamic acid was not in the vacuole fraction. The possible role of the vacuole on the biosynthesis of capsaicinoid is also discussed.

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