Production, Purification and Characterization of HYI, an Antiyeast Substance, Produced by Hansenula saturnus

Abstract

An anti-yeast substance, HYI, was produced by Hansenula saturnus. Production of HYI under aerobic incubation conditions took place in parallel with yeast cellular growth. HYI was concentrated by ultra filtration from the culture filtrate and purified by ion-exchange chromatography followed by gel filtration. It was then crystallized. HYI migrated as a single protein band on sodium dodecyl sulfate/polyacrylamide gel electrophoresis and had a molecular weight of about 8500. Anthrone positive compounds could not be detected in HYI. The isoelectric point ofHYI was pH 5.80±0.05. The activity ofHYI was unaffected by heating at 60°C for 1 hr. It showed a broad anti-yeast spectrum.