Occurrence of a New Enzyme, meso-Tartrate Dehydratase of Pseudomonas putida

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A new enzyme that catalyzes the dehydration of meso-tartrate was found in the cell-free extract of Pseudomonas putida isolated from soil. The bacterial cells also produce D-tartrate dehydratase which acts on D-tartrate specifically. The meso-tartrate dehydratase is inducibly produced on the addition of meso-tartrate to the medium, and is very unstable. The enzyme is specific for meso-tartrate, other isomers not acting as substrates. The enzyme requires no cofactor, although it is inhibited by oxygen and thiols. The properties of the enzyme differ from those of D- and L-tartrate dehydratases from pseudomonads reported previously.