Abstract
The ddh gene of Corynebacterium glutamicum encoding meso-diaminopimelate (meso-DAP)-Ddehydrogenase (DDH) involved in the lysine biosynthesis was cloned in a DAP auxotroph (dap D4) of Escherichia coli by complementation of the DAP auxotroph. Deletion analysis revealed that a - 1.7-kb XhoI-KpnI fragment contained the ddh structure gene. The specific activity of DDH was increased fourteen-fold when C. glutamicum was transformed with a recombinant plasmid harbouring the cloned ddh gene. Furthermore, the ddh gene has been sequenced [S. Ishino et al., Nucleic Acids Res., 15, 3917 (1987)] and some properties of the ddh gene are discussed.
