A Novel Sulfatase from Pseudomonas testosteroni Hydrolyzing Lithocholic Acid Sulfate

Abstract

Pseudomonas testosteroni ATCC 11996 was found to produce a novel bile acid sulfate sulfatase that hydrolyzes the sulfate ester bond in lithocholic acid sulfate (LCA-S). The enzyme synthesis was induced by several kinds of bile acids including LCA-S. Mn²⁺ functioned as an essential component for the enzyme synthesis and SO^2-₄ suppressed it. This sulfatase hydrolyzes LCA-S to isolithocholic acid and sulfuric acid with inversion of α- to β-configuration of the hydroxyl group at the third position of lithocholic acid.