Regeneration of Protoplasts Prepared from Alkaliphilic Strains of Bacillus spp.

Abstract

Protoplasts from four alkaliphilic strains of Bacillus spp. regenerated their cell walls and grew on a hypertonic medium. The regeneration was effective at neutral pH but not at alkaline pH. The protoplasts seemed not to be tolerant of the alkaline pH that is optimum for growth of the strains.