1994 Volume 58 Issue 11 Pages 2066-2067
A simple and rapid method was developed separating phosphoserine, phosphothreonine, phosphotyrosine, phosphoric acid, and ATP by one-dimensional cellulose thin-layer chromatography, using a freshly prepared solvent system of 1-butanol-formic acid-water (5 : 2 : 1). This system was applied to determine phosphoamino acid (phosphoserine) of wheat elongation factor 1β phosphorylated by casein kinase II from the wheat embryo.
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