Isolation of Soybean 11S Globulin by Isoelectric Precipitation and Sephacryl S-300 Gel Filtration Chromatography : A New Purification Technique

Abstract

This study outlines a simple alternative technique for the purification of the 11S soybean globulin from crude globulin using a single Sephacryl S-300 gel filtration chromatographic technique. All supporting data demonstrated that the 11S globulin was purified to homogeneity with no contamination from the 7S soybean globulin.