Transformation of Acetobacter polyoxogenes with Plasmid DNA by Electroporation

Abstract

Acetobacter polyoxogenes was transformed with plasmid DNA by electroporation. The following points were essential for transformation: (i) dilution of the culture broth with cold water and air bubbling of the culture broth for transformation at discharging from a jar fermentor, and (ii) selection of transformants by liquid cultivation. For shortening of the lag time in cultivation for selection of transformants, the following treatments were useful: (i) addition of sucrose to the cell suspension during transformation and to the broth for cultivation, and (ii) addition of 1mM MgCl₂ to a mixture of cells and DNA during electroporation.