Enzymatic Peptide Synthesis with Fatty Acid-modified Chymotrypsin and Trypsin in Aqueous-organic Media

Abstract

Equilibrium controlled enzymatic peptide syntheses in aqueous-organic media were done with chymotrypsin and trypsin modified with varied fatty acid. Palmitoyl-modified chymotrypsin and trypsin synthesized the peptide at 1. 7 and 2. 4 times higher yield than those unmodified. Since an alteration of the substrate specificity was not observed, the chemical modification did not affect the active site of the enzymes.