Formation of Hydroxyl Radicals in the Stems of Winter Wheat Treated with Freezing Temperature

Abstract

Levels of hydroxyl radicals were measured by the methanesulfinic acid (MSA) formed from dimethyl sulfoxide in winter wheat stems treated at a critical low temperature. The level of MSA reached more than 0. 3 μmol per g fresh weight upon incubation at - 20°C. These results suggest the formation of hydroxyl radicals cause the death of plants.