Some Properties of Transglycosylation Activity of Sesame β-Glucosidase

Abstract

The transglycosylation reaction of partially purified β-glucosidase from sesame seeds with cellobiose is described. Sesame β-glucosidase was partially purified by ammonium sulfate fractionation and gel filtration. The molecular weight of the enzyme was 200, 000 by gel filtration. Sesame β-glucosidase showed strong transfer activity to synthesize the trisaccharide from cellobiose. The optimum pH and temperature of the transglycosylation reaction were pH 4.0 and 60°C.