Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Variation of Inhibition Modes by n-Alcohols in Arbutin (Hydroquinone-O-β-D-glucopyranoside) Hydrolysis by β-Glucosidase
Noriko SakamaKoji Tadasa
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JOURNAL FREE ACCESS

1995 Volume 59 Issue 7 Pages 1352-1354

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Abstract

When n-alcohols were added to the β-glucosidase-catalyzed hydrolysis of arbutin, the rate was decreased almost linearly with their elevating concentrations. The inhibition was shown in two modes, competitive for higher n-alcohols and noncompetitive for lower n-alcohols than n-butanol. The inhibition constants (Ki) obtained, moreover, clearly demonstrated classifying the modes in two groups according to physicochemical parameters, logP, Hildebrand solubility parameter (δ), and dielectric constant (ε), of n-alcohols. n-Butanol being between two modes of inhibition showed a mixed-type inhibition. The data suggested that the inhibition of n-alcohols for β-glucosidase may be related with their chain length. An imaging model in this case of inhibition is presented.

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