Purification and Characterization of a Lectin from Amaranthus hypochondriacus var. Mexico Seeds

Munetaka OZEKI; Kazuo KAMEMURA; Kikuko MORIYAMA; Yayoi ITOH; Yukio FURUICHI; Hayato UMEKAWA; Takao TAKAHASHI

doi:10.1271/bbb.60.2048

Munetaka OZEKI, Kazuo KAMEMURA, Kikuko MORIYAMA, Yayoi ITOH, Yukio FURUICHI, Hayato UMEKAWA, Takao TAKAHASHI

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Keywords: Amaranthus, lectin, hemagglutinin, purification

JOURNAL FREE ACCESS

1996 Volume 60 Issue 12 Pages 2048-2051

DOI https://doi.org/10.1271/bbb.60.2048

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Abstract

A lectin from Amaranthus hypochondriacus var. Mexico (AHML) was purified by affinity chromatography using asialofetuin-Sepharose 4B. AHML is specific for N-acetyl-D-galactosamine as are the other Amaranthus lectins. AHML has no carbohydrate moiety and requires no metal ion for the hemagglutination activity. The pI of AHML is 6.8. AHML has a native molecular mass of 45.0 kDa and is composed of homo-subunits having molecular masses of 36.8 kDa.

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