Inhibitory Effects of Caffeic Acid Ethyl Ester on H2O2-induced Cytotoxicity and DNA Single-strand Breaks in Chinese Hamster V79 Cells

Tsutomu NAKAYAMA; Munetaka YAMADA; Toshihiko OSAWA; Shunro KAWAKISHI

doi:10.1271/bbb.60.316

Inhibitory Effects of Caffeic Acid Ethyl Ester on H₂O₂-induced Cytotoxicity and DNA Single-strand Breaks in Chinese Hamster V79 Cells

Tsutomu NAKAYAMA, Munetaka YAMADA, Toshihiko OSAWA, Shunro KAWAKISHI

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Keywords: hydrogen peroxide, cultured cell, DNA strand break, caffeic acid ethyl ester, polyphenol

JOURNAL FREE ACCESS

1996 Volume 60 Issue 2 Pages 316-318

DOI https://doi.org/10.1271/bbb.60.316

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Abstract

Cytotoxicity and DNA single-strand breaks caused by H₂O₂were assessed by a colony formation assay and a DNA precipitation assay, respectively, with Chinese hamster V79 cells. In both assays, caffeic acid ethyl ester showed protective effects. The structure-activity relationship showed that the o-dihydroxy structure of caffeic acid ethyl ester was essential for the protective effects.

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