Determination of Glutathione and Related Aminothiols in Mouse Tissues by Gas Chromatography with Flame Photometric Detection

Abstract

A selective and sensitive method for the determination of glutathione and such related aminothiols as Cys, cysteinylglycine and γ-glutamylcysteine in mouse tissues by gas chromatography was developed. After fractionating a tissue sample into the free and protein-bound fractions, glutathione and aminothiols in each fraction were converted inito their N, S-isopropoxycarbonyl methyl ester derivatives and then measured by gas chromatography with flame photometric detection, using a DB-1 capillary column. This method enabled the redox status of glutathione and related aminothiols in mouse tissues to be investigated.