1996 Volume 60 Issue 8 Pages 1351-1352
Compared with the enzyme activity of a recombinant Taka-amylase A (wild TAA) at 25°C and pH 5.3, those of two mutants (E230Q and D297N) were 1/10, 000 and 1/16, 000 for amylase activity, and 1/920 and less than 1/20, 000 for maltosidase activity, respectively. This indicates that all residual activities of E230Q are not due to contamination by wild-type TAA. The results from difference spectroscopy suggested that E230Q retains amylose binding ability.
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