Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Changes of Lipoxygenase and Fatty Acid Hydroperoxide Lyase Activities in Bell Pepper Fruits during Maturation
Jun-ichi KajiharaKozue ShibataKazuo Kato
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JOURNAL FREE ACCESS

1997 Volume 61 Issue 1 Pages 199-201

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Abstract

Human urokinase (UK) was easily degraded during the incubation at 37°C in a time-dependent manner. The degradation was also observed in the presence of trypsin inhibitors, suggesting that UK was not degraded by exogeneous trypsin-like serine protease but by autolysis. In this cases, the A-chain of UK was selectively degraded. Polyethylene glycol-polypropylene glycol conjugated urokinase (PEG-PPG-UK) was not degraded after prolonged incubation at 37°C. These results demonstrated that PEG-PPG modification completely blocked the degradation of UK by autolysis.

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