Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
High-level Expression of Maize C4-type Phosphoenolpyruvate Carboxylase in Escherichia coli and Its Rapid Purification
Long-Ying DONGShingo HATAKatsura IZUI
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JOURNAL FREE ACCESS

1997 Volume 61 Issue 3 Pages 545-546

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Abstract

Maize C4-type phosphoenolpyruvate carboxylase (PEPC) was expressed in E. coli with the pET32 system. The expressed fusion PEPC was active and its amount comprised more than 10% of total soluble protein. The specific activity inbreased by about 45-fold, compared with our previous system [S. Yanagisawa and K. Izui, Agric. Biol. Chem., 54, 241-243 (1990)]. The fusion PEPC was rapidly purified with His bind metal chelation resin, showing a single band on SDS-PAGE. Moreover, the tag domain fused at the N-terminus did not have any effect on catalytic and regulatory properties of PEPC.

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