Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean

Abstract

Mannosylated derivatives of cyclodextrins (CDs), mannosyl-α, β, and γCD were synthesized from a mixture of mannose and α, β, and γCD by the reverse action of α-mannosidase from jack bean, respectively. Their structures were analyzed by FAB-MS and ¹³C-NMR spectroscopies, and they were identified as 6-O-α-D-mannosyl-α, β, and γCD. The optimum conditions for the production of 6-O-α-D-mannosyl-αCD by α-mannosidase were examined. Optimum pH and temperature were pH 4.5 and 60°C, respectively. Yield of mannosyl-αCD increased with increasing mannose concentration and reached more than 35% (mol/mol) at the concentration of 2 M mannose and 0.4 M αCD.