Bulletin of the Chemical Society of Japan
Online ISSN : 1348-0634
Print ISSN : 0009-2673
ISSN-L : 0009-2673
Verification of Conformation Change in Ca2+-Bound S-100 Proteins Caused by Mg2+-Binding
Sadayuki Matsuda
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2002 Volume 75 Issue 11 Pages 2503-2507

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Abstract

The conformation changes of S-100a.a′ and S-100b caused by Mg2+-binding were determined by several methods. The fluorescence intensity of the Ca2+-bound S-100a.a′ was enhanced by the Mg2+-binding. The conformation changes in Ca2+/S-100a.a′ and Ca2+/S-100b caused by Mg2+-binding were also detected using a fluorescence environmental probe, 2-p-toluidinonaphthalene-6-sulfonate (TNS). The reactivities of the cysteine (Cys) residues in S-100a.a′ and S-100b to a thiol-specific reagent, 2,2′-dinitro-5,5′-dithiodibenzoic acid (DTNB), were increased by the Mg2+-binding, regardless of the Ca2+-binding.

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© 2002 The Chemical Society of Japan
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