2002 Volume 75 Issue 11 Pages 2503-2507
The conformation changes of S-100a.a′ and S-100b caused by Mg2+-binding were determined by several methods. The fluorescence intensity of the Ca2+-bound S-100a.a′ was enhanced by the Mg2+-binding. The conformation changes in Ca2+/S-100a.a′ and Ca2+/S-100b caused by Mg2+-binding were also detected using a fluorescence environmental probe, 2-p-toluidinonaphthalene-6-sulfonate (TNS). The reactivities of the cysteine (Cys) residues in S-100a.a′ and S-100b to a thiol-specific reagent, 2,2′-dinitro-5,5′-dithiodibenzoic acid (DTNB), were increased by the Mg2+-binding, regardless of the Ca2+-binding.
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