Bulletin of the Chemical Society of Japan
Online ISSN : 1348-0634
Print ISSN : 0009-2673
ISSN-L : 0009-2673
The Fabrication of an Amperometric Immunosensor Based on Double-Layer 2D-Network (3-Mercaptopropyl)trimethoxysilane Polymer and Platinum-Prussian Blue Hybrid Film
Lingyan ZhangKun Qi
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2018 Volume 91 Issue 3 Pages 368-374


A label-free amperometric immunosensor using Prussian blue as an artificial peroxidase to detect methamphetamine was developed. The immunosensor exhibited excellent stability even in neutral and weakly alkaline conditions. Platinum-Prussian blue (Pt-PB) hybrid film co-deposited on an electrode was coated with double-layer 2D-network 3-mercaptopropyl-trimethoxysilane (3-MPS), the thiols on which were utilized to absorb nano-Au in order to capture antibody. The Pt nanoparticle in the hybrid film acted not only as a carrier of PB to stabilize the PB film, but also a modifier for catalytic function because it increased the size of the lattice channels in PB film. Meanwhile, the double-layer 2D-network 3-MPS film could help stabilizing the Pt-PB film with its densely-packed structure and its homogeneous and defect-free characteristics. The signal readout was obtained electrochemically via PB-catalyzed H2O2 evolution reaction, which was inversely proportional to the concentration of methamphetamine in the sample. Thus, methamphetamine could be detected in 1.0 × 10−8 M–4.0 × 10−6 M by the immunosensor.

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© 2018 The Chemical Society of Japan
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