1953 Volume 26 Issue 6 Pages 294-298
Tobacco mosaic virus (TMV) was purified by differential centrifugation in a vacuum-type, airdriven ultracentrifuge, first constructed in Japan.
The yield of purified TMV was high and about 2 mg. per cc. of the original plant juice.
Chemical analyses and physico-chemical examinations (including electrophoresis, sedimentation, diffusion, viscosity and electron microscope measurements) were carried out on the purified preparations to test their purities and homogeneities. It was found that the purity of ultracentrifugally isolated TMV was very high in a chemical sense. Although the homogeneity withrespect to the length of particles was not always so good, one preparation, TMV-9, was found to have a high degree of homogeneity by the sedimentation and electrophoresis patterns. The size and shape of TMV particles were determined on this preparation by several methods.
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