Bulletin of the Chemical Society of Japan
Online ISSN : 1348-0634
Print ISSN : 0009-2673
ISSN-L : 0009-2673
Effects of Lysine and Arginine Derivatives on the Base Stacking Association of 2′-Deoxyguanosine 5′-Monophosphate
Kazumi HirotaMitsuru IgarashiYoshio InoueRiichirô Chûjô
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1983 Volume 56 Issue 12 Pages 3796-3801

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Abstract

The effects of basic amino acid residues on the base stacking of nucleotides have been studied to clarify the nature of interaction between histones and DNA′s. For this purpose, lysine and arginine derivatives were used as models for basic amino acid residues in histone. The base stacking association of 2′-deoxyguanosine 5′-monophosphate(5′-dGMP) in aqueous solution has been studied by the measurement of the concentration dependence of 1H NMR chemical shifts of 5′-dGMP both in the absence and in the presence of the basic amino acid derivatives at different pH’s. We clarified that the lysine favors the base stacking association of 5′-dGMP through the interaction with the phosphate group(s), while an excess of the arginine destabilizes the stacking aggregates through the interaction with the base moiety. We also clarified that the ionization of the phosphate group destabilizes the stacking association of 5′-dGMP both in the absence and in the presence of the basic amino acids.

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