1934 Volume 9 Issue 6 Pages 263-268
A new micro-method for the determination of R—S–S–R (Cystine) and R–SH (Cysteine) is described, based on the fact that dimethyl-p-phenylenediamine hydrochloride gives a distinct methylene blue colour with R—SH compounds when the proper concentration of acid and of ferric ammonium sulphate are used. The colour thus obtained is permanent.
The advantages of this method are ease in determination, lack of interfering substances under normal biological conditions, and relative equivalence of R—SH and R—S–S–R groups.
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