Bulletin of the Chemical Society of Japan
Online ISSN : 1348-0634
Print ISSN : 0009-2673
ISSN-L : 0009-2673
A MICRO-METHOD FOR THE DETERMINATION OF DISUL-PHIDE-AND SULFHYDRYL-COMPOUNDS. I
Hideo Toyoda
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1934 Volume 9 Issue 6 Pages 263-268

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Abstract

A new micro-method for the determination of R—S–S–R (Cystine) and R–SH (Cysteine) is described, based on the fact that dimethyl-p-phenylenediamine hydrochloride gives a distinct methylene blue colour with R—SH compounds when the proper concentration of acid and of ferric ammonium sulphate are used. The colour thus obtained is permanent.
The advantages of this method are ease in determination, lack of interfering substances under normal biological conditions, and relative equivalence of R—SH and R—S–S–R groups.

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