Chromosome Preparations from Larvae and Adult Tissues of Polychaetes

MINORU IKEDA; MASANORI SATO

doi:10.5179/benthos1990.1991.41_29

MINORU IKEDA, MASANORI SATO

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JOURNAL FREE ACCESS

1991 Volume 1991 Issue 41 Pages 29-35

DOI https://doi.org/10.5179/benthos1990.1991.41_29

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Published: September 01, 1991 Received: February 13, 1991 Available on J-STAGE: August 07, 2009 Accepted: June 14, 1991 Advance online publication: - Revised: -
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Date of correction: August 07, 2009 Reason for correction: - Correction: CITATION Details: Wrong : BERTOUT, M., 1976. Spermatogenèse de Nereis diversicolor O. F. MÜLLER (Annéride Polychète) 1. évolution du cytoplasme et élaboration de l'acrosome. J. Microsc. Biol. Cell., 25:87-94.
CHRISTENSEN, B., 1980. Animal Cytogenetics, Vol. 2. Annelida. Gebruder Borntraeger, Berlin, 81 PP.
GEORGE, J. D. and G. HARTMANN-SCHRÖDER, 1985. Polychaetes : British Amphinomida, Spintherida and Eunicida. E. J. Brill/Dr. W. Buckhuys, London, 221 pp.
GRASSLE, J. P., C. E. GELFMAN and S. W. MILLS,1987. Karyotypes of Capitella sibling species, and of several species in the related genera Capitellides and Capitomastus (Polychaeta). Bull. Biol. Soc. Washington, 7:77-88.
IMAI,H.T.,R.H.CROZIER and R.W.TAYLOR, 1977. Karyotype evolution in Australian ants. Chromosoma, 59:341-393.
KLIGERMAN, A. D. and S. E. BLOOM, 1977. Rapid chromosome preparations from solid tissues of fishes. J. Fish. Res. Board Can., 34 : 266-269.
MACGREGOR, H. and J. VARLEY, 1983. Working with Animal Chromosomes. Second edition. John Wiley & Sons, Chichester, 290pp.
MUROFUSHI, M. and Y. DEGUCHI, 1983. A method for obtaining metaphase chromosomes from large shrimp-like crustaceans. Rep. Mishima Res. Inst. Sci. Liv. Nikon Univ., 6:31-34.
OLIVE, P. J. W., 1983. Annelida - Polycheata. In, Reproductive Biology of Invertebrates, Vol. 2 : Spermatogenetics and Sperm Function, ed. by K. G. ADIYODI and R. G. ADIYODI, John Wiley & Sons, Chichester, pp. 321-342.
PESCH, G. G. and C. E. PESCH, 1980. Chromosome complement of the marine worm Neanthes arenaceodentata (Polycheata : Annelida). Can. J. Fish. Aquat. Sci., 37:286-288.
PESCH, G. G., C. MUELLER and C. E. PESCH, 1988. Chromosome of the marine worm Nephtys incisa (Annelida : Polychaeta). Ophelia, 28:157-161.
PESCH, G. G., C. E. PESCH and A. R. MALCOLM, 1981. Neanthes arenaceodentata, a cytogenetic model for marine genetic toxicology. Aquatic Toxicology, 1:301-311.
PESCH, G. G., C. E. PESCH and C. MUELLER, 1988. Chromosome complements from two popula. tions of the marine worm Neanthes arenaceodentata (Annelida : Polychaeta). Ophelia, 28:163-167.
ROTHFELS, K. H. and L. SIMINOVITCH, 1958. An air-drying technique for flattening chromo. somes in mammalian cells grown in vitro. Stain TechnoL 33:73-77.
TJIO, J. H. and T. T. PUCK, 1958. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J. Exp. Med. 108:259-268.
YAMAZAKI, F., H. ONOZATO and K. ARAI, 1981. The chopping method for obtaining permanent chromosome preparation from embryos of teleost fishes. Bull. Japan. Soc. Sci. Fish., 47:963.

Right : BERTOUT, M., 1976. Spermatogenèse de Nereis diversicolor O. F. MÜLLER (Annéride Polychète) 1. évolution du cytoplasme et élaboration de l'acrosome. J. Microsc. Biol. Cell., 25:87-94.
CHRISTENSEN, B., 1980. Animal Cytogenetics, Vol. 2. Annelida. Gebruder Borntraeger, Berlin, 81 PP.
GEORGE, J. D. and G. HARTMANN-SCHRÖDER, 1985. Polychaetes : British Amphinomida, Spintherida and Eunicida. E. J. Brill/Dr. W. Buckhuys, London, 221 pp.
GRASSLE, J. P., C. E. GELFMAN and S. W. MILLS,1987. Karyotypes of Capitella sibling species, and of several species in the related genera Capitellides and Capitomastus (Polychaeta). Bull. Biol. Soc. Washington, 7:77-88.
IMAI,H.T.,R.H.CROZIER and R.W.TAYLOR, 1977. Karyotype evolution in Australian ants. Chromosoma, 59:341-393.
KLIGERMAN, A. D. and S. E. BLOOM, 1977. Rapid chromosome preparations from solid tissues of fishes. J. Fish. Res. Board Can., 34 : 266-269.
MACGREGOR, H. and J. VARLEY, 1983. Working with Animal Chromosomes. Second edition. John Wiley & Sons, Chichester, 290pp.
MUROFUSHI, M. and Y. DEGUCHI, 1983. A method for obtaining metaphase chromosomes from large shrimp-like crustaceans. Rep. Mishima Res. Inst. Sci. Liv. Nikon Univ., 6:31-34.
OLIVE, P. J. W., 1983. Annelida - Polycheata. In, Reproductive Biology of Invertebrates, Vol. 2 : Spermatogenetics and Sperm Function, ed. by K. G. ADIYODI and R. G. ADIYODI, John Wiley & Sons, Chichester, pp. 321-342.
PESCH, G. G. and C. E. PESCH, 1980. Chromosome complement of the marine worm Neanthes arenaceodentata (Polycheata : Annelida). Can. J. Fish. Aquat. Sci., 37:286-288.
PESCH, G. G., C. MUELLER and C. E. PESCH, 1988. Chromosome of the marine worm Nephtys incisa (Annelida : Polychaeta). Ophelia, 28:157-161.
PESCH, G. G., C. E. PESCH and A. R. MALCOLM, 1981. Neanthes arenaceodentata, a cytogenetic model for marine genetic toxicology. Aquatic Toxicology, 1:301-311.
PESCH, G. G., C. E. PESCH and C. MUELLER, 1988. Chromosome complements from two popula. tions of the marine worm Neanthes arenaceodentata (Annelida : Polychaeta). Ophelia, 28:163-167.
ROTHFELS, K. H. and L. SIMINOVITCH, 1958. An air-drying technique for flattening chromo. somes in mammalian cells grown in vitro. Stain TechnoL 33:73-77.
TJIO, J. H. and T. T. PUCK, 1958. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J. Exp. Med. 108:259-268.
YAMAZAKI, F., H. ONOZATO and K. ARAI, 1981. The chopping method for obtaining permanent chromosome preparation from embryos of teleost fishes. Bull. Japan. Soc. Sci. Fish., 47:963.

Abstract

Methods for preparations of mitotic chromosomes are described for use on polychaetes. Well-spread metaphases are available by using larvae (trochophores, metatrochophores and nectochaetes) and adult tissues (clumps of spermatogonia and regenerating posterior ends). Principal procedures of the preparations consist of the following steps : 1) Pretreatment of the materials with a 0.005-0.05% colchicine solution. 2) Immersion in a hypotonic solution (1% sodium citrate). 3) Fixation in a freshly prepared fixative of methanol-acetic acid (1:1 or 3: 1). 4) Spreading of cells on a glass slide. 5) Air-drying or flame-drying. 6) Staining with a 2 % solution of Giemsa.

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