Abstract
Germfree and conventionalized rats were fed a basal or 2% phytate diet. On mucosal phytase activity, the intestinal microflora has no marked effect. Dietary phytate decreased phytase activity in mucosal homogenates, but this effect was not clear in the course of purification. In the germfree intestinal mucosa, the electrophoretic pattern showed two peaks of phytase activity which were different in divalent metal ion requirements. One of these purified phytases did not have any alkaline phosphatase activity.
