Restriction Fragment Length Polymorphism in Helicobacter pylori Associated with Gastric Cancer and Duodenal Ulcer

Koichi KIMURA; Shunji HAYASHI; Hiroshi ISOGAI; Emiko ISOGAI; Toshiro SUGIYAMA; Masahiro ASAKA; Tomonori ANBO; Akimichi IMAMURA; Takeshi NISHIKAWA; Toru KUBOTA; Nobuhiro FUJII

doi:10.12938/bifidus1996.17.61

Restriction Fragment Length Polymorphism in Helicobacter pylori Associated with Gastric Cancer and Duodenal Ulcer

Koichi KIMURA, Shunji HAYASHI, Hiroshi ISOGAI, Emiko ISOGAI, Toshiro SUGIYAMA, Masahiro ASAKA, Tomonori ANBO, Akimichi IMAMURA, Takeshi NISHIKAWA, Toru KUBOTA, Nobuhiro FUJII

Author information

Keywords: Helicobacter pylori, RFLP, vacA, gastric cancer, duodenal ulcer

JOURNAL FREE ACCESS

1998 Volume 17 Issue 1 Pages 61-64

DOI https://doi.org/10.12938/bifidus1996.17.61

Details

Abstract

More than 90% of 75 clinical isolates of Helicobacter pylori strains had the vacuolating cytotoxin gene vacA as demonstrated by polymerase chain reaction (PCR). A PCR restriction fragment-length polymorphism (RFLP) analysis revealed various gene subtypes, and some of the RFLPs correlated significantly with gastric cancer or duodenal ulcer. These findings suggest that specific types of H. pylori have factors that cause gastric cancer or duodenal ulcer.

Corresponding author

Register with J-STAGE for free!