Examination of Conditions for External Quality Control in Identification of Microorganisms using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

KAZUYUKI SOGAWA; KOHEI UECHI; MAKIKO KIYOSUKE; SYOTA MURATA; KOJI KUSABA; YUJI SAEKI; KAZUKI HORIUCHI; KAZUNARI YASUDA; HAJIME OKUMURA; AZUMI FUJINAGA; MASAMI MURAKAMI

doi:10.4265/bio.27.179

Note

Examination of Conditions for External Quality Control in Identification of Microorganisms using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

KAZUYUKI SOGAWA, KOHEI UECHI, MAKIKO KIYOSUKE, SYOTA MURATA, KOJI KUSABA, YUJI SAEKI, KAZUKI HORIUCHI, KAZUNARI YASUDA, HAJIME OKUMURA, AZUMI FUJINAGA, MASAMI MURAKAMI

Author information

KAZUYUKI SOGAWA
Department of Biochemistry, School of Life and Environmental Science, Azabu University
KOHEI UECHI
Department of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus
MAKIKO KIYOSUKE
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Hospital
SYOTA MURATA
Department of Clinical Laboratory, Chiba University Hospital
KOJI KUSABA
Department of Laboratory, Saga university Hospital
YUJI SAEKI
Clinical Laboratory, University of Miyazaki Hospital
KAZUKI HORIUCHI
Department of Laboratory Medicine, Shinshu University Hospital
KAZUNARI YASUDA
Clinical Laboratory, Mie University Hospital
HAJIME OKUMURA
bioMérieux Japan Ltd.,
AZUMI FUJINAGA
Bruker Japan K.K.,
MASAMI MURAKAMI
Clinical Laboratory Center, Gunma University Hospital

Keywords: External quality control, Identification of microorganisms, Matrix-assisted laser desorption, ionization time-of-flight mass spectrometry

JOURNAL FREE ACCESS

2022 Volume 27 Issue 3 Pages 179-184

DOI https://doi.org/10.4265/bio.27.179

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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was approved for medical use in 2011 and is currently used as a rapid, accurate and lowcost technique for bacterial identification. External quality control for medical analysis is monitored using tests of the Japanese Association of Medical Technologists and Prefectural Association of Clinical Laboratory Technologists and through user surveys of reagent and equipment manufacturers. However, external quality control of bacterial typing using MS is not performed. Therefore, we examined procedures for evaluating quality control of bacterial typing using an identification reliability index at 38 facilities.

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