2005 年 10 巻 1-2 号 p. 73-77
A rapid and simple procedure for the specific detection of Salmonella was developed by using a dissolved oxygen measurement device (DOX-96) with anti-Salmonella antibodies. In the DOX-96 system, a gold electrode is located at the bottom of each well, in a 96-hole plate. The gold electrode acts as the working electrode. The anti-Salmonella antibodies are then introduced into the system and immobilized on each well of the plate. Wells contained bound Salmonella Typhimurium cells which were incubated at 37°C, and the oxygen consumption in each well was monitored. It appeared that the oxygen consumption curve was inversely proportional to the growth of S. Typhimurium. In the present method, S. Typhimurium cells with an initial concentration of 2.5 ×10 0-2.5 ×108CFU/ml in the sample showed an oxygen consumption curve within 13 h of incubation. Other microorganisms, such as Escherichia coli, Pseudomonas aeruginosa, Corynebacterium aquaticum and Bacillus subtilis did not interfere with the assay system. Thus the present method would be applicable toward a rapid and simple detection of Salmonella in food.
