The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
The Structure of the O-Specific Chain of Lipopolysaccharide from Pseudomonas aeruginosa IID 1012 (ATCC 27588)
Shunji KayaYoshio ArakiEiji Ito
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1989 Volume 105 Issue 1 Pages 29-34

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Abstract

Structural studies were carried out on the O-polysaccharide fraction obtained from the lipopolysaccharide of Pseudomonas aeruginosa IID 1012, the standard strain of Homma serogroup K, by mild acid treatment. The O-polysaccharide was composed of L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-D-galactosaminuronic acid. The results from analysis of fragments obtained by acid hydrolysis and Smith degradation of the O-polysaccharide, together with data on methylation analysis and nuclear magnetic reso-nance spectroscopic measurement of the polysaccharide, led to the most likely structure of the repeating units of the polymer chain, →4)D-GalNAcA(α1→3)D-QuiNAc(β1→2)L-Rha(αl→3)L-Rha(αl→, in which about 20% of the N-acetylgalactosaminuronic acid resi-dues were in an amide form and about 75% of the same residues were O-acetylated at C-3.

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