The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Post-Translational Import of 3-Ketoacyl-CoA Thiolase into Rat Liver Peroxisomes In Vitro
Satoshi MiuraShoko MiyazawaTakashi OsumiTakashi HashimotoYukio Fujiki
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1994 Volume 115 Issue 6 Pages 1064-1068

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Abstract

Cell-free translation products of hepatic free polysomal RNA from a clofibrate-treated rat were incubated at 26°C for 0-60 min with a post-heavy mitochondrial supernatant fraction from normal rat liver. Exogenously added proteinase K-resistant precursor and mature forms of peroxisomal 3-ketoacyl-CoA thiolase were recovered in a particulate fraction and increased with time. Both forms of thiolase cosedimented with peroxisomes, when the proteinase K-treated import reaction mixture was centrifuged in a sucrose density gradient. The in vitro import and processing of thiolase precursors, types A and B, was likewise reproduced with highly purified peroxisomes. These results strongly suggest that the precursor form of 3-ketoacyl-CoA thiolase is translocated into peroxisomes, apparently without tight coupling with proteolytic processing to the mature protein.

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