Crystallization and Preliminary X-Ray Analysis of a Truncated Family A Alkaline Endoglucanase Isolated from Bacillus sp. KSM-635

Tsuyoshi Shirai; Takashi Yamane; Teruo Hidaka; Kazuo Kuyama; Atsuo Suzuki; Tamaichi Ashida; Katsuya Ozaki; Susumu Ito

Crystallization and Preliminary X-Ray Analysis of a Truncated Family A Alkaline Endoglucanase Isolated from Bacillus sp. KSM-635

Tsuyoshi Shirai, Takashi Yamane, Teruo Hidaka, Kazuo Kuyama, Atsuo Suzuki, Tamaichi Ashida, Katsuya Ozaki, Susumu Ito

Author information

Tsuyoshi Shirai
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Takashi Yamane
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Teruo Hidaka
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Kazuo Kuyama
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Atsuo Suzuki
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Tamaichi Ashida
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University
Katsuya Ozaki
Tochigi Research Laboratories of Kao Corporation
Susumu Ito
Tochigi Research Laboratories of Kao Corporation

Keywords: alkaline cellulase, crystallization, detergent enzyme, endo-1, 4-β-glucanase, X-ray crystallography

JOURNAL FREE ACCESS

1997 Volume 122 Issue 4 Pages 683-685

Details

Abstract

The catalytic domain of an alkaline endo-1, 4-β-glucanase (family A) isolated from Bacillus sp. KSM-635 (M_r=40.2 kDa) was crystallized using the hanging drop vapor diffusion method. Two different crystal forms were obtained. Form 2 crystals (trigonal space group R3 with cell dimensions of a=b=111.9 and c=207.1 Å in a hexagonal lattice) were found to be more stable than form 1 ones upon X-ray irradiation. A full data set for form 2 crystals has been collected up to 3.3 Å resolution.

Corresponding author

Register with J-STAGE for free!