1997 Volume 122 Issue 4 Pages 683-685
The catalytic domain of an alkaline endo-1, 4-β-glucanase (family A) isolated from Bacillus sp. KSM-635 (Mr=40.2 kDa) was crystallized using the hanging drop vapor diffusion method. Two different crystal forms were obtained. Form 2 crystals (trigonal space group R3 with cell dimensions of a=b=111.9 and c=207.1 Å in a hexagonal lattice) were found to be more stable than form 1 ones upon X-ray irradiation. A full data set for form 2 crystals has been collected up to 3.3 Å resolution.