Cloning of a Rat Glia Maturation Factor-γ (rGMFG) cDNA and Expression of Its mRNA and Protein in Rat Organs

Hideki Tsuiki; Kiyofumi Asai; Manami Yamamoto; Kaori Fujita; Yuichiro Inoue; Yoko Kawai; Toyohiro Tada; Akihiko Moriyama; Yoshiro Wada; Taiji Kato

Hideki Tsuiki, Kiyofumi Asai, Manami Yamamoto, Kaori Fujita, Yuichiro Inoue, Yoko Kawai, Toyohiro Tada, Akihiko Moriyama, Yoshiro Wada, Taiji Kato

著者情報

キーワード: gene cloning, gene expression, Northern blot analysis, rat glia maturation factor-γ, Western blot analysis

ジャーナルフリー

2000 年 127 巻 3 号 p. 517-523

詳細

抄録

We isolated a rat glia maturation factor-γ (rGMFG) cDNA and examined the tissue distribution of GMFG in rat by Northern and Western blot analyses. Sequence analysis of the entire cDNA revealed an open reading frame of 426 nucleotides with a deduced protein of 142 amino acid residues. The deduced amino acid sequence of the putative product is highly homologous (78.9%) to rat glia maturation factor-β (rGMFB). Northern blot analysis indicated that a 0.9-kb mRNA is predominantly expressed in rat thymus, testis, and spleen. GMFG showed a different tissue distribution from GMFB, being present predominantly in proliferative and differentiative organs.

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